Queen Beryl- The evil Queen of the Dark Kingdom. She is gathering energy from people in order to rule the universe. She has hired four lieutenants to help her complete her mission. She was destroyed by Princess Serenity using the power of the Ginshouzou. In Japan, her voice is done by Han Keiko.

Jadeite- Queen Beryl's first lieutenant. He usually disguised himself as different humans in his plots. He hardly accomplished anything in his missions; he witnessed two other senshi (Mars and Mercury) joining the group. He was permanently frozen in ice by Queen Beryl after failing too many times. In Japan, his voice is done by Onosaka Masaya.

Nephrite- Queen Beryl's second lieutenant. He disguised himself as Sanjouin Masato and manipulated humans by complementing them on certain abilities. Naru fell in love with him, and showed him what love meant. Nephrite was killed by Zoisite and his minions because he was a traitor to the Dark Kingdom. In Japan, his voice is done by Katsuji Mori.

Zoisite- Queen Beryl's third lieutenant. In the North American series, he was turned into a she because Zoisite and Kunzite were lovers. Zoisite initially gathered 4 out of the 7 Rainbow Crystals, and later was able to retrieve the rest in a duel with Tuxedo Kamen. He was also very stubborn, and questioned Queen Beryl's orders at times. He tried to kill Tuxedo Kamen, and when Beryl found out, she killed him. In Japan, his voice is done by Nanba Keiichi.